Plasmid_Backbone

Part:BBa_J72208:Design

Designed by: Josh Kittleson   Group: Anderson Lab   (2012-09-17)


Kanamycin resistant BAC with R6K origin


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 6909
    Illegal XbaI site found at 6924
    Illegal SpeI site found at 1
    Illegal PstI site found at 2383
    Illegal PstI site found at 3924
    Illegal PstI site found at 5450
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 6909
    Illegal SpeI site found at 1
    Illegal PstI site found at 2383
    Illegal PstI site found at 3924
    Illegal PstI site found at 5450
    Illegal NotI site found at 391
    Illegal NotI site found at 6915
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 6909
    Illegal BglII site found at 2732
    Illegal BglII site found at 4803
    Illegal BglII site found at 5237
    Illegal XhoI site found at 4936
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a suffix.
    Illegal SpeI site found at 1
    Illegal PstI site found at 2383
    Illegal PstI site found at 3924
    Illegal PstI site found at 5450
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 6909
    Plasmid lacks a suffix.
    Illegal XbaI site found at 6924
    Illegal SpeI site found at 1
    Illegal PstI site found at 2383
    Illegal PstI site found at 3924
    Illegal PstI site found at 5450
    Illegal NgoMIV site found at 247
    Illegal NgoMIV site found at 257
    Illegal NgoMIV site found at 4242
    Illegal NgoMIV site found at 5901
    Illegal AgeI site found at 1995
    Illegal AgeI site found at 2888
    Illegal AgeI site found at 4118
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1141
    Illegal BsaI.rc site found at 6072
    Illegal BsaI.rc site found at 6638
    Illegal BsaI.rc site found at 6816
    Illegal SapI.rc site found at 354
    Illegal SapI.rc site found at 2138
    Illegal SapI.rc site found at 3348
    Illegal SapI.rc site found at 5750
    Illegal SapI.rc site found at 5960


Design Notes

n/a


Source

n/a

References